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. 2017 Nov 29;10:56. doi: 10.1186/s13041-017-0334-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overexpression of active or inactive PKMζ in cultured rat hippocampal neurons and single molecule imaging of GluA2-containing AMPARs. a Experimental workflow. b Transgene expression using TetON system. c Representative image of fluorescence microscopy of Homer1C-fused mCerulean3.0 and anti-protein kinase C, zeta (PKCζ) immunofluorescence signals in neurons expressing active PKMζ (T227E), inactive PKMζ (K98W), or neither (CTL). d Representative image of Qdot-labelled GluA2-containing AMPARs (red, maximum intensity projection of 500 frames) and mCerulean3.0 (cyan, average image of 100 frames). (e) Qdot trajectories (open circles) observed in the dendritic region (cyan)