Table I.
Primer pairs used in polymerase chain reactions.
| Primer pair | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| EXONS 1–5 | CCACAAGCACCTTCAGTCCAG | CAAAGGAAGGAATGTCCATGTCTA |
| 5′-RACE 1 | GCCACGCGTCGACTAGTACGGGGGGGGGG | GCCAGTGCAACCCTGCACTTGTAAA |
| 5′-RACE 2 | GGCCACGCGTCGACTAGTAC | TCTTCAGTTTTATCTGTGGAGGTGGGTTCT |
| 3′-RACE 1 | TCCCACCCCTGACCCAACTTCAAAT | GCTGTCAACGATACGCTACGTAAC |
| 3′-RACE 2 | TCAAGAGGAGTGGCTAAAGAAGGTGACAGT | GCTACGTAACGGCATGACAGTG |
| Genome 1 | ACGCTTATACTTTTAGTTCTTCATGGA | CAGCTATGCCAGGCAGTGTTT |
| Genome 2 | TGTGTCTGAGTTTTAAGCTGCCA | GGCAGAATGAAGCACATCCAA |
| Genome 3 | CTGGAGGAGAAGAAATTGTGGAG | TCCTTCTAGCTCTTTCTTAGGCAA |
| Genome 4 | CAACCCAGAGGGACCAATTTT | CAGGCCATTCATTTCCTTTCT |
| Genome 5 | AGTGCCAAAGTCCAAGGGTC | TCTGTCCTTGAGCCCACCA |
| GAPDH | CCATCACCATCTTCCAGGAGCGAG | CAAAGGTGGAGGAGTGGGTGTCG |
| IL-6 qPCR | CCTGACCCAACTTCAAATGC | CACACTACATTAGCCGAGAAGC |
| GAPDH | CCATCACCATCTTCCAGGAGCGAG | CAAAGGTGGAGGAGTGGGTGTCG |
RACE, rapid amplification of mRNA ends; IL-6, interleukin-6; qPCR, quantitative polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.