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. 2017 Sep 28;40(6):1699–1708. doi: 10.3892/ijmm.2017.3158

Figure 1.

Figure 1

Autophagy in PC12 cells following IH. (A) Autophagosomes (green) in PC12 cells at various OGD treatment times (LC3 immunolabeling). IH induced autophagy in the PC12 cells. The number of autophagosomes increased with OGD in a time-dependent manner. Scale bar, 30 µm. Expression of (B) LC3-II, p62 and (C) Beclin 1, determined using western blot analysis. The expression levels of LC3-II and Beclin 1 increased in a time-dependent manner, and the expression of p62 decreased in time-dependent manner. Grayscale ratios of LC3-II, p62 and Beclin 1 (target protein/β-actin) are shown in histograms. (D) Expression of LC3-II increased significantly from OGD 6 h in a time-dependent manner, (E) expression of p62 decreased from OGD 0.5 h in a time-dependent manner (F) Expression of Beclin 1 was similar to that of LC3-II. OGD groups differed significantly from the control with time-dependency. *P<0.05 and **P<0.01 vs. control group. Data are expressed as the mean ± standard error of the mean of four independent experiments. IH, ischemia-hypoxia; OGD, oxygen-glucose deprivation; LC3, microtubule-associated protein 1A/1B-light chain 3; Con, control.