Figure 2.

Inhibitory effects of black rice anthocyanins (BRACs) on cell migration and invasion. (A) The migration of cells was analyzed using a wound healing assay. Cells were observed under a microscope following treatment with or without BRACs (0 or 200 µg/ml). (B) The invasion of cells was assessed by an 8-µm pore size cell culture insert. Cells were treated with or without BRACs (0 or 200 µg/ml) for 24 h, followed by counting of the invading cells in 5 different visual fields counted from 4 independent experiments. (C) Migration distances were measured using ImageJ software. Values are presented as the means ± SD of 4 replicated experiments. ^*P<0.05 denotes a significant difference from the control. (D) Invading cells are shown as the means ± SD of 4 replicated experiments. ^*P<0.05 denotes a significant difference from the control.