Figure 5.

The chondrocyte-like ATDC5 cells were co-treated with IL-1β (10 ng/ml) and different concentrations of ISL (2.5, 5 and 10 µmol/l) for 48 h. The COL II, COX-2 and MMP-13 mRNA and protein expression was detected by (A) reverse transcription-quantitative polymerase chain reaction and western blotting (B), respectively. GAPDH was used as internal control. The results represent the mean ± standard deviation of three independent experiments. ^*P<0.05 vs. model group treated with only IL-1β. IL, interleukin; ISL, isoliquiritigenin; COL, collagen; COX, cyclooxygenase; MMP, matrix metalloproteinase.