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. 2017 Sep 29;40(6):1631–1638. doi: 10.3892/ijmm.2017.3167

Figure 1.

Figure 1

Hypothermia inhibits the proliferation and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and increases their tolerance to hypoxia. (A) Detection of CD71, CD105 (BMSC markers), CD34 and CD45 (hematopoietic stem cell markers) in BMSCs by flow cytometry. (B) Growth curve of BMSCs. (C) Cell cycle analysis of BMSCs by flow cytometry. (D) Differentiation of BMSCs into nerve cells detected by immunofluorescence (scale bar, 25 µm). (E and F) Apoptosis detection by flow cytometry (scale bar, 50 µm). (G) Measurement of LDH content by enzyme-linked immunosorbent assay (ELISA). *p<0.05 and **p<0.01, compared with the 37°C normal group; △p<0.05, compared with the 37°C OGD group,.