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. 2017 Sep 29;40(6):1631–1638. doi: 10.3892/ijmm.2017.3167

Figure 2.

Figure 2

Hypothermia induces SUMOylation and small ubiquitin-like modifier (SUMO) nuclear transfer in bone marrow-derived mesenchymal stem cells (BMSCs). (A) Detection of SUMO1 levels in covalent and free states by western blot analysis. (B) Detection of SUMO2/3 levels in covalent and free states by western blot analysis. (C) Quantification of data in panel A. (D) Quantification of data in panel B. (E) A shift of SUMO1 and the SUMO2/3 complex from the cytoplasm to the nucleus was observed by immunofluorescence (scale bar, 10 µm). Red, SUMO1 protein; green, SUMO2/3 protein; blue, DAPI labeling of nuclei. *p<0.05, **p<0.01.