| optimize recovery of urine volumes |
use cotton wool in diapers to collect urine |
|
include water and urine blank samples in analytical run and apply database of known contaminants to identify chemical contaminants arising from diaper or cotton wool |
|
use modified centrifugation technique for extraction |
| minimization of chemical contaminants |
use same brand of collection kit (cotton wool, diapers) |
|
use centrifugation to minimize extraction of contaminants and maximize volume |
| optimization of sampling handling |
use only one freeze–thaw cycle |
|
establish clear SOP for biofluid collection and preparation |
| optimization of spectral preprocessing |
normalize spectral data to the median spectrum |
|
remove spectral features corresponding to known chemical contaminants prior to modeling the data |
| age variability |
detailed records/metadata relating to demographic factors including age, post menstrual age, and feeding when possible, impose a narrow age range inclusion criteria |
