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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Eur J Immunol. 2017 Jul 31;47(9):1525–1534. doi: 10.1002/eji.201747062

Fig. 5. Optimization of CD1b-MA tetramer loading.

Fig. 5

a CD1b monomers were treated with methoxy MA at several molar ratios in buffers containing the indicated percentages of CHAPS detergent, tetramerized using streptavidin-allophycocyanin, and used to stain the indicated T cell line or clone. b Using a 5:1 molar ratio and 0.6% CHAPS, methoxy-, α-, or keto MA were loaded into CD1b tetramers and used to stain the indicated T cell line or clone. Stainings in b and c were performed twice independently. Staining of GEM18 was performed three times independently.