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Full-length H1.0 was cloned into the pGEX-4T-1 vector with a GST fusion at the N-terminus and 6xHis-tag at the C-terminus. Globular H1.0 (H1g) and C-terminal H1.0 (H1-C) were generated by iPCR from full-length H1.0 with an N-terminal 6xHis-SUMO-HRV 3C site fusion in the pETDuet-1 vector.
