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(A): plasmid construct (pNeo4MTT1) used for MTT1KO strain. (B): plasmid construct (pNeo4MTT5) used for MTT5KD. (C): plasmid construct (pCHXMTT5) used for obtaining MTT1KO + MTT5KD. neoTet: neomycin/paromomycin resistance gene. rpl29: cycloheximide resistance gene. BTU2 3': part of the 3'UTR region of the β-tubulin 2 gene.
