Fig 2. Increased expression of CAV3 promoted a shift of GLUT4.
The expression of CAV3 and GLUT4 was detected by confocal microscopy (magnification: 40×). Representative photomicrographs show C2C12 cells transfected with an empty vector and with WT-CAV3, followed by double staining to detect CAV3 (purple) and GLUT4 (red). Nuclei ere stained blue with DAPI. Cells were transfected with eGFP (green). Transfection of CAV3 increased the level of CAV3 protein and promoted a shift of GLUT4 to the cell membrane. Scale bars = 50 μm.