Table 7. Parameters of intra- and interspecific variation of Aneura pinguis based on K2P (%) model of nucleotide substitution.
DNA region | N | Mean | Mean | Median | Min | Max | Overlap1 | Percentile | Percentile | Overla2 | Percentile | Percentile | Overla3 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
inter- | ||||||||||||||
/mean intraspecific | 10% | 90% | 5% | 95% | ||||||||||
intra | 792 | 0.14 | 0.05 | 0.00 | 0.99 | 0.00 | 0.25 | 0.00 | 0.86 | |||||
matK | inter | 4564 | 4.96 | 35× | 5.60 | 0.86 | 8.45 | 0.13 | 1.98 | 6.95 | 0 | 1.61 | 7.21 | 0 |
intra | 576 | 0.18 | 0.16 | 0.00 | 0.49 | 0.00 | 0.33 | 0.00 | 0.49 | |||||
rbcL | inter | 3165 | 2.51 | 14× | 2.52 | 0.66 | 4.44 | 0 | 1.16 | 3.37 | 0 | 0.82 | 3.90 | 0 |
intra | 148 | 0.25 | 0.14 | 0.00 | 1.23 | 0.00 | 1.08 | 0.00 | 1.09 | |||||
rpoC1 | inter | 1077 | 3.92 | 16× | 4.04 | 0.68 | 6.39 | 0.55 | 1.49 | 5.89 | 0 | 1.09 | 6.09 | 0 |
intra | 390 | 0.38 | 0.13 | 0.00 | 1.40 | 0.00 | 1.02 | 0.00 | 1.15 | |||||
trnH-psbA | inter | 2536 | 4.35 | 11× | 4.85 | 1.14 | 6.66 | 0.26 | 2.31 | 5.60 | 0 | 1.66 | 5.79 | 0 |
intra | 792 | 0.21 | 0.08 | 0.00 | 0.95 | 0.00 | 0.76 | 0.00 | 0.76 | |||||
trnL-trnF | inter | 4564 | 4.58 | 22× | 5.16 | 0.95 | 7.39 | 0 | 2.30 | 6.35 | 0 | 1.34 | 7.16 | 0 |
intra | 576 | 0.16 | 0.14 | 0.00 | 0.63 | 0.00 | 0.28 | 0.00 | 0.63 | |||||
matK+rbcL | inter | 3165 | 3.91 | 24× | 4.34 | 0.85 | 6.24 | 0 | 1.77 | 5.17 | 0 | 1.41 | 6.33 | 0 |
intra | 792 | 0.16 | 0.07 | 0.00 | 0.90 | 0.00 | 0.37 | 0.00 | 0.75 | |||||
matK+trnL-trnF | inter | 4564 | 4.81 | 30× | 5.74 | 1.05 | 7.85 | 0 | 1.96 | 6.62 | 0 | 1.58 | 7.11 | 0 |
intra | 390 | 0.25 | 0.12 | 0.00 | 0.75 | 0.00 | 0.62 | 0.00 | 0.69 | |||||
matK+trnH-psbA | inter | 2536 | 4.68 | 18× | 5.30 | 1.25 | 7.11 | 0 | 2.35 | 6.17 | 0 | 1.70 | 6.62 | 0 |
intra | 576 | 0.24 | 0.17 | 0.00 | 0.70 | 0.00 | 0.61 | 0.00 | 0.61 | |||||
rbcL+trnL-trnF | inter | 3165 | 3.63 | 15× | 3.97 | 0.88 | 5.95 | 0 | 1.86 | 4.90 | 0 | 1.23 | 5.18 | 0 |
intra | 390 | 0.25 | 0.12 | 0.00 | 0.75 | 0.00 | 0.62 | 0.00 | 0.69 | |||||
rbcL+trnH-psbA | inter | 2536 | 4.68 | 12× | 5.30 | 1.25 | 7.11 | 0 | 2.02 | 6.34 | 0 | 1.70 | 6.62 | 0 |
intra | 338 | 0.24 | 0.14 | 0.00 | 0.71 | 0.00 | 0.69 | 0.00 | 0.69 | |||||
combined cp loci | inter | 1542 | 4.14 | 17× | 4.56 | 1.03 | 6.40 | 0 | 1.81 | 5.60 | 0 | 1.38 | 6.15 | 0 |
intra | 792 | 0.94 | 0.29 | 0.00 | 3.91 | 0.00 | 3.29 | 0.00 | 3.60 | |||||
ITS1 | inter | 4564 | 11.94 | 13× | 12.50 | 0.88 | 18.59 | 3.03 | 4.58 | 16.58 | 0 | 3.91 | 17.72 | 0 |
intra | 792 | 0.80 | 0.41 | 0.00 | 3.35 | 0.00 | 2.49 | 0.00 | 2.50 | |||||
ITS2 | inter | 4564 | 10.02 | 12× | 11.57 | 0.82 | 16.85 | 2.53 | 2.49 | 13.94 | 0 | 1.23 | 14.62 | 0.0127 |
intra | 792 | 0.74 | 0.14 | 0.00 | 3.08 | 0.00 | 2.51 | 0.00 | 2.65 | |||||
ITS | inter | 4564 | 8.92 | 12× | 9.91 | 0.96 | 13.64 | 2.12 | 2.80 | 12.11 | 0 | 2.37 | 12.79 | 0.0028 |
intra | 338 | 0.31 | 0.09 | 0.00 | 1.04 | 0.00 | 0.97 | 0.00 | 0.99 | |||||
combined data set | inter | 1542 | 4.78 | 15.4× | 5.12 | 1.28 | 7.45 | 0 | 1.96 | 6.60 | 0 | 1.47 | 6.88 | 0 |
Note: Overlap1 = Maximum of intraspecific—Minimum of interspecific distances; Overlap2 = 90% of intraspecific—10% of interspecific distances; Overlap3 = 95% of intraspecific—5% of interspecific distances.