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. 2017 Nov 2;45(21):12585–12598. doi: 10.1093/nar/gkx1007

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Specific growth rate of strains expressing FnCpf1 and their control strains. A: IMX1139, expressing FnCpf1 constitutively from its genomic DNA, and its congenic control strain CEN.PK113–7D. B: IME384, expressing FnCpf1 from a multicopy plasmid (pUDE731) and its congenic control strain IME385 containing the same multicopy plasmid but without FnCpf1 (pUD706). The strains were cultivated in shake-flask on chemically defined medium with glucose as sole carbon source. The data points represent the average and mean deviation of two independent culture replicates.