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. 2017 Nov 2;45(21):12585–12598. doi: 10.1093/nar/gkx1007

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Efficiency of ADE2 editing by FnCpf1. (A) Design of the CRISPR plasmid harbouring the CRISPR array for in vivo assembly in yeast. SHR, homologous sequence for recombination (43). (B) AT content and position in the coding region of ADE2 of the crRNAs. (C) Comparison of the genome editing efficiency of six crRNA with various AT content and target sequence (grey bars). The genome editing efficiency was also measured when cells were incubated after transformation in liquid medium for 48 h (black bars). The efficiency is calculated as the number of red colonies divided by the total number of colonies on the transformation plates in the presence of repair DNA fragments. Values represent the average and standard deviation of two biological and two technical replicates. (Plasmids used: pUD605 to pUD609, Table 2).