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Confirmation of FnCpf1-mediated introduction of a point mutation in ADE2. Sanger sequencing of the genomic DNA locus targeted for FnCpf1-mediated point mutation in seven randomly selected transformants. The control is the genomic DNA of the congenic strain CEN.PK113–7D. (Plasmid used: pUD628 carrying crADE2–3.S, Table 2).
