Figure 5.

FnCpf1-mediated editing of single and double genomic targets. (A) Composition of CRISPR arrays for single and double deletion of ADE2 and HIS4. 19-nt direct repeats were used and CRISPR plasmids were assembled in vitro using Gibson assembly. (B) Fraction of transformants with single or double deletion as measured by diagnostic PCR (Supplementary Figure S5), following the design described in A. The number of transformants checked by PCR is indicated between brackets. (Plasmids used: pUD628, pUDE712 to pUDE714, pUDE708 to pUDE710). Plating was performed just after transformation, without additional incubation.