Table 3. Attributes of the spacers used in this study.
| Targeted gene | crRNA name | 5′ to 3′ sequence (PAM) | AT content (%) | Position from ATG |
|---|---|---|---|---|
| ADE2 (1716 nt) | crADE2–1 | T(TTA)CGGGCACACCGATGACAGGAAGTGG | 36 | 1438 |
| crADE2–2 | T(TTT)CGGCGTACAAAGGACGATCCTTCAG | 44 | 723 | |
| crADE2–3 | T(TTC)CCGGTTGTGGTATATTTGGTGTGGA | 52 | 743 | |
| crADE2–4 | T(TTA)CATTCAATTGTGCAAATGCCTAGAG | 60 | 1498 | |
| crADE2–5 | T(TTA)ATTTGGGATGTTTTACTTGAAGATT | 72 | 247 | |
| crADE2–6 | T(TTG)ATTAAATGCTCTTTTTGAATATATT | 84 | 317 | |
| CAN1 (1773 nt) | crCAN1–1 | T(TTA)TTTGGTCTATCAAAGAACAAGTTGG | 64 | 1204 |
| crCAN1–2 | CTT(TTC)ATTGGTTTATCCACACCTCTGACCA | 64 | 322 | |
| crCAN1–3 | CAT(TTC)AAGGTACTGAACTAGTTGGTATCAC | 60 | 893 | |
| crCAN1–4 | GTT(TTG)CCACATATCTTCAACGCTGTTATCT | 60 | 1123 | |
| HIS4 (2400 nt) | crHIS4–1 | G(TTG)CCCAATGTAAGGAGATTGTGTTTGC | 56 | 1514 |
| crHIS4–2 | T(TTC)TCCAATCAATTCATGGTAAAACAAA | 72 | 328 | |
| crHIS4–3 | T(TTA)CTAAAGATTCTAGCCCCACCAAACC | 52 | 730 | |
| crHIS4–4 | T(TTA)GCATCTTGGCTAGCAATGAACAGAG | 52 | 227 | |
| PDR12 (4536 nt) | crPDR12–1 | A(TTC)CATTTATGAAATATGAAGCTGGTGC | 64 | 1847 |
| crPDR12–2 | CAT(TTC)GTCGAGATCGAACCATGACGATGAT | 52 | 39 | |
| crPDR12–3 | GTT(TTA)GCACAAAGAATCAATATGGGTGTCA | 60 | 2674 | |
| crPDR12–4 | CAT(TTC)GCATATAAGCATGCTTGGAGAAATT | 62 | 2269 |
NB: in the text and in Table 2, a letter is added at the end of the crRNA name listed in this table to indicate whether the crRNA is framed by short (S, 19 nt) or long (L, 36 nt) direct repeats.