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. 2017 Dec 5;6:e31649. doi: 10.7554/eLife.31649

Figure 2. Smo activation prevents obesity and improves glucose metabolism in mice on high fat diet.

(A) A schematic for experimental design. (B) Representative images after 8 weeks on HFD. (C) Measurements of body weight. (D) Body composition after 8 weeks on HFD. (E, F) Whole-mount images (upper) and histology (lower) of gonadal white fat (E) or interscapular brown fat (F) after 8 weeks on HFD. (G) Detection of bone marrow fat by perilipin immunofluorescence staining after 8 weeks on HFD. Boxed regions shown at higher magnification in insets. (H, I) Gene expression by qPCR in WAT (H) and BAT (I) after 8 weeks on HFD. (J) Gli1 expression in different tissues after 8 weeks on HFD. (K, L) Glucose tolerance test (GTT) (K) and insulin tolerance test (ITT) (L) after 8 weeks on HFD. *p<0.05, n = 5 mice, females. Males show similar results. Black scale bar: 100 μm; white scale bar: 200 μm.

Figure 2.

Figure 2—figure supplement 1. No obvious effect for Dox or Pparg-tTA alone on whole body metabolism.

Figure 2—figure supplement 1.

(A–B) Body weight (A) and body composition (B) of wild-type mice (C57BL/6) fed with HFD and with or without Dox water starting at 2 months of age for two additional months. N = 3, males. (C–E) Body composition (C), body weight (D) and GTT (E) of Pparg-tTA mice raised on Dox from conception till 2 months of age before being fed with HFD and with or without Dox water for two additional months. N = 3, males.
Figure 2—figure supplement 2. Characterization of adipogenesis in M2 cells.

Figure 2—figure supplement 2.

The mRNA levels of early (A) and late (B) adipogenic genes were determined by qPCR from 6 hr through 72 hr in response to the adipogenic media (AdipoM) versus the growth media (Ctrl). 18S was used as the internal control. *p<0.05, n = 3 (biological replicates).
Figure 2—figure supplement 3. Hh signaling suppresses adipogenesis in M2 cells.

Figure 2—figure supplement 3.

(A) Representative images of oil red O staining in M2 cells cultured in AdipoM with or without PM. (B, C) PM suppresses expression of Pparg, Cebpa and Fabp4 but not Cebpb or Cebpd. 18S was used as the internal control for all qPCR analyses. *p<0.05, n = 3 (biological replicates).