Figure 3. lncRNA-SVUGP2 inhibits proliferation of HepG2 and Hep3B human liver cancer cell lines.

(A) RT-qPCR was used to measure the level of lncRNA-SVUGP2 in control or lncRNA overexpression (OE) group in both HepG2 and Hep3B liver cancer cell line. For control group, cells were infected with lentivirus produced by PGMLV-PA6 empty vector. Cells of OE group were infected with lentivirus encoding lncRNA-SVUGP2. (B) Cell proliferation rate of control and OE group was detected by cck-8 assay in both HepG2 and Hep3B cells. (C) EdU cell proliferation assay showing overexpression of lncRNA-SVUGP2 suppress cell proliferation. In this assay, EdU is incorporated into cellular DNA, which further reacts with fluorescent reagents. (D) To detect cell proliferation rates in vivo, we established a xenograft model in nude mice. Control or lncRNA-overexpressing HepG2 or Hep3B cells were injected subcutaneously into nude mice. Five weeks after injection, mice were sacrificed, and tumors were photographed and weighed. qPCR (E) and western blot (F) was used to detect the mRNA and protein level of Ki-67 and PCNA, two growth markers, in control and lncRNA-overexpressing cells. ^*p < 0.05, ^**p < 0.01, relative to control group.