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. 2017 May 16;8(58):97928–97940. doi: 10.18632/oncotarget.17880

Figure 3. PLK1 inhibition induces a G2/M arrest in (pre)cancerous cells, together with loss of bipolar spindle formation.

Figure 3

(A) VU-preSCC-M3 and UM-SCC-22A as a reference, were exposed to BI6727 and GSK461364. This resulted in a strong G2/M arrest for both cell lines. Primary keratinocytes (PK) showed a less pronounced G2/M arrest after drug treatment. Bars represent the percentage of cells in a certain phase as indicated. Mean percentages of three independent experiments are shown. Data was analyzed with the Poisson equation and demonstrated a significant difference between the cell lines and the primary cells, for both GSK461364 and BI6727 (P<0.001). (B) Similar as to the inhibitors, VU-preSCC-M3 cells transfected with the siRNA SMARTpool directed against PLK1 undergo a G2/M arrest. Bars represent the percentages of cells similar as indicated in A. (C) VU-preSCC-M3 cells were treated with 0.07 μM GSK461364 and visualization of α-tubulin showed disorganized monopolar spindles in almost all cells. In contrast, untreated cells displayed only normal bipolar arranged spindles. Representative pictures of untreated and treated cells are shown, scale bars indicate 25 μm.