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. 2017 Aug 16;8(58):97990–98003. doi: 10.18632/oncotarget.20296

Figure 7. The effect of TSC-22 in the CSF-1R autocrine loop.

Figure 7

(A) HeLa cells were transfected with TSC-22 in a time-dependent manner. Whole cell lysates were used in western blotting. The expression levels of TSC-22, CSF-1R, CSF-1, and GAPDH are shown. (B) HeLa cells were transfected with control siRNA or TSC-22 siRNA for 24 hours and then transfected with the TSC-22 expression vector for 24 hours. Protein levels of CSF-1R and TSC-22 were determined by western blot assay. (C) Cell lysates were immunoprecipitated with TSC-22 or CSF-1R antibody and immunoblotted with CSF-1R, TSC-22, or CSF-1. (D) HeLa cells were transiently transfected with pcDNA4-TSC-22 and pcDNA4-CSF-1. An injury line was made on the confluent monolayer of cells. Cellular migration was observed with a light microscope (×40) at the indicated time points. The width of the injury line was measured and plotted on a graph.