Skip to main content
. 2017 Aug 16;8(58):97990–98003. doi: 10.18632/oncotarget.20296

Table 1. Primers used in cloning of yeast two-hybrid assay.

Gene Location Forward/reverse Primer sequence Restriction site
TSC-22 Full 1-144 Forward 5’-CGGGAATTCATGAAATCCCAATGGTGT-3‘ EcoR I
Reverse 5’-CGGCTCGAGCTATGCGGTTGGTCCTGA-3’ Xho I
1-52 Forward 5’-CGGGAATTCATGAAATCCCAATGGTGT-3’ EcoR I
Reverse 5’-ATTCTCGAGTCAGTCAATAGCTACCAC-3’ Xho I
53-110 Forward 5’-CGGGAATTCATGAACAAAATCGAGCAAGCT-3’ EcoR I
Reverse 5’-ATTCTCGAGTCAAAACTGGGCAAGCTG-3’ Xho I
111-144 Forward 5’-CGGGAATTCATGCAGGCCCAGCTGCAGACT-3’ EcoR I
Reverse 5’-CGGCTCGAGCTATGCGGTTGGTCCTGA-3’ Xho I
CSF-1R Full 1-972 Forward 5’-CGGGAATTCATGGGCCCAGGAGTTC-3’ EcoR I
Reverse 5’-ATGCGGATCCTCAGCAGAACTGATA-3’ BamH I
1-538 Forward 5’-CGGGAATTCATGGGCCCAGGAGTTC-3’ EcoR I
Reverse 5’-ATGCGGATCCTCAGTACAATAGCAG-3’ BamH I
539-972 Forward 5’-ATGCGAATTCATGAAGTATAAGCAG-3’ EcoR I
Reverse 5’-ATGCGGATCCTCAGCAGAACTGATA-3’ BamH I
679-918 Forward 5’-ATGCGAATTCATGGAGGCCATGCTG-3’ EcoR I
Reverse 5’-ATGCGGATCCTCACTCCTGTCCTCT-3’ BamH I
750-918 Forward 5’-ATGCGAATTCATGGAGCTCCGGGAC-3’ EcoR I
Reverse 5’-ATGCGGATCCTCACTCCTGTCCTCT-3’ BamH I
919-972 Forward 5’-ATGCGAATTCATGGAGCGGGACTAT-3’ EcoR I
Reverse 5’-ATGCGGATCCTCAGCAGAACTGATA-3’ BamH I