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. 2017 Oct 3;8(58):98103–98116. doi: 10.18632/oncotarget.21463

Table 2. A list of primers in qRT-PCR analysis of mRNA expression of the target genes.

Genes GenBank accession Primer sequence(5’→3’) Product size
NF-κB NM205134 Forward: TCAACGCAGGACCTAAAGACAT 162 bp
Reverse: GCAGATAGCCAAGTTCAGGATG
TNF-α NM204267 Forward: GCCCTTCCTGTAACCAGATG 71 bp
Reverse: ACACGACAGCCAAGTCAACG
PTGES NM001194983 Forward: GTTCCTGTCATTCGCCTTCTAC 115 bp
Reverse: CGCATCCTCTGGGTTAGCA
COX-2 NM001167718 Forward: TGTCCTTTCACTGCTTTCCAT 84 bp
Reverse: TTCCATTGCTGTGTTTGAGGT
iNOS NM204961 Forward: CCTGGAGGTCCTGGAAGAGT 82 bp
Reverse: CCTGGGTTTCAGAAGTGGC
IL-1β NM204524 Forward: CAGCAGCCTCAGCGAAGAG 86 bp
Reverse: CTGTGGTGTGCTCAGAATCCA
IL-2 AF033563 Forward: TTCAAAATATCGAAAAGAACCTCAAG 51 bp
Reverse: CGGTGTGATTTAGACCCGTAAGAC
IL-6 NM204628 Forward: AAATCCCTCCTCGCCAATCT 106 bp
Reverse: CCCTCACGGTCTTCTCCATAA A
IL-8 NM205498 Forward: GGCTTGCTAGGGGAAATGA 199 bp
Reverse: AGCTGACTCTGACTAGGA AACTGT
IL-12β NM213571 Forward: TGTCTCACCTGCTATTTGCCTTAC 87 bp
Reverse: CATACACATTCTCTCTAAGTTTCCACTGT
IL-17 AY744450 Forward: CATGTTGTCAGCCAGCATTTCT 107bp
Reverse: CATCTTTTTGGGTTAGGCATCC
IFN-γ GQ246226 Forward: GTGAAGAAGGTGAAAGATATCATGGA 71 bp
Reverse: GCTTTGCGCTGGATTCTCA
IL-4 AJ621249 Forward: GTGCCCACGCTGTGCTTAC 82 bp
Reverse: AGGAAACCTCTCCCTGGATGTC
IL-10 AJ621614 Forward: CGCTGTCACCGCTTCTTCA 88bp
Reverse: TCCCGTTCTCATCCATCTTCTC
HSP27 NM205290 Forward: ACACGAGGAGAAACAGGATGAG 158 bp
Reverse: ACTGGATGGCTGGCTTGG
HSP40 NM001199325 Forward: GGGCATTCAACAGCATAGA 151 bp
Reverse: TTCACATCCCCAAGTTTAGG
HSP60 NM001012916 Forward: AGCCAAAGGGCAGAAATG 208 bp
Reverse: TACAGCAACAACCTGAAGACC
HSP70 NM001006685 Forward: CGGGCAAGTTTGACCTAA 250bp
Reverse: TTGGCTCCCACCCTATCTCT
HSP90 NM001109785 Forward: TCCTGTCCTGGCTTTAGTTT 143 bp
Reverse: AGGTGGCATCTCCTCGGT
β-actin NM205518 Forward: CCGCTCTATGAAGGCTACGC 128 bp
Reverse: CTCTCGGCTGTGGTGGTGAA