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. 2017 Oct 5;8(58):98200–98214. doi: 10.18632/oncotarget.21516

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Copyright: © 2017 Neuber et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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The MNC of 4 HDs were incubated with peptide-loaded DC in the presence of lenalidomide (1 μM and 10 μM) or pomalidomide (0.1 μM, 1 μM) or without lenalidomide/pomalidomide as the negative control (Contr.). After incubation for 4 hrs, 16 hrs, 24 hrs, 72 hrs, 14 d and 21 d, the cells were stained for CD3, CD8 and KPNA2 and were analyzed by flow cytometry. Figure 6 shows the gating strategy (A) and the KPNA2-expression of CD3⁺CD8⁺ T-cells as representative histogram from 4 HDs (after 4 hrs, B) and as cumulative results from 4 HDs (C).

The MNC of 4 HDs were incubated with peptide-loaded DC in the presence of lenalidomide (1 μM and 10 μM) or pomalidomide (0.1 μM, 1 μM) or without lenalidomide/pomalidomide as the negative control (Contr.). After incubation for 4 hrs, 16 hrs, 24 hrs, 72 hrs, 14 d and 21 d, the cells were stained for CD3, CD8 and KPNA2 and were analyzed by flow cytometry. Figure 6 shows the gating strategy (A) and the KPNA2-expression of CD3⁺CD8⁺ T-cells as representative histogram from 4 HDs (after 4 hrs, B) and as cumulative results from 4 HDs (C).