Figure 1. NGS data of snakeheads and analysis data during screening the sex-specific molecular markers.

DNAs of M1, F1, F’-Mix were used for DNA sequencing. Libraries were separately run in three lane of an Illumina HiSeq 2000, using 150 base paired-end reads. The raw reads were filtered, and obtained clean reads. 196 candidate Y chromosome-specific fragments were screened from the NGS data through bioinformatics analysis, such as genome assembly and alignments.