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. 2017 Oct 19;8(58):98733–98744. doi: 10.18632/oncotarget.21924

Figure 3. PCR validation results of three pairs of sex-specific molecular markers in commonly wild-caught 48 XY males and 48 XX females (M1-M48 and F1-F48, respectively).

Figure 3

(A) A 237 bp male-specific fragment (indicated by arrow) was amplified in all male individuals by 359642-F/R primer pair. (B) A 158 bp male-specific fragment (indicated by arrow) was amplified in all male individuals by 418354-F/R primer pair. (C) A 303 bp male-specific fragment (indicated by arrow) was amplified in all male individuals and multiple bands in all female individuals by 275834X/Y-F primer pair, M: DL2000 DNA marker.