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. 2017 Oct 19;8(58):98733–98744. doi: 10.18632/oncotarget.21924

Figure 4. PCR amplification results of homologous cloning on X chromosome.

Figure 4

(A) The PCR amplification results of 275834X/Y-F and 275834X/Y-R in 48 males and 48 females (M1-M48 and F1-F48, respectively). A 458 bp band was expected from PCR amplification in both sexes. Male individuals were also expected to have a 570 bp band that was Y chromosome-specific. (B) Sequence alignments of Y chromosome-specific fragment with its homologous fragment on X chromosome. 275834-Y represented the sex-specific fragment on Y chromosome. 275834-X represented the fragment on X chromosome. The red area in the blue line represented the bases which did not map. Red solid line represented the unique regions of 275834-X, and red dotted line represented the deletions of the homologous fragment on X chromosome. The purple arrows were on behalf of the loci of pair primer: 275834X/Y-F and 275834X/Y-R, and the green arrows were on behalf of the loci of pair primer: 275834-F and 275834-R.