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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Prog Biophys Mol Biol. 2017 Aug 6;130(Pt B):244–253. doi: 10.1016/j.pbiomolbio.2017.07.011

Figure 1.

Figure 1

TRPV 2 and 4 are not activated in the patch dome. Patches were formed from myotubes, and visualized, mechanically stimulated and recorded from as in [38]. Myotubes were transfected with either C-terminally tagged TRPV2 (A) or TRPV4 (B). Fluorescence images next to DIC images show channel proteins present in the patch domes of both cell-attached and excised inside-out patches. Patches were stretched with 500 ms negative pressure steps and the mean integrated current during the pressure steps are shown in (C). Patch currents from myotubes expressing TRPV2 and TRPV4 were not significantly different from control cell patches.