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. Author manuscript; available in PMC: 2018 Dec 15.
Published in final edited form as: J Immunol. 2017 Nov 1;199(12):3943–3951. doi: 10.4049/jimmunol.1700211

Figure 1. The model system of eosinophilic and neutrophilic airway inflammation.

Figure 1

B6 mice were either injected i.p. with CA/Alum or s.c. with CA/CFA as described in Supplementary Fig S1. Two weeks later mice were intranasally challenged for 4 consecutive days with CA in PBS. Mice were sacrificed 24 hours after the last challenge. (A) Cells from BAL were stained for Ly6G and Siglec F expression. (B) Differential cell count of BAL cells was performed using FACS. (C) Mice were sacrificed at different time points as shown in Supplementary Fig S1. BAL cells were then stained for eosinophils, neutrophils, and CD4 T cells. (D and E) Draining mediastinal LN (D) and Lung (E) cells were collected and ex vivo stimulated to measure intracellular cytokine expression. (F) Draining LN cells were ex vivo stimulated with CA or OVA protein for 3 days. Cytokine secretion was determined by ELISA. Each symbol represents individually tested mice. The data shown are the mean ± s.d. of three independent experiments. *, p<0.05; **, p<0.01; ***, p<0.001.