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. Author manuscript; available in PMC: 2018 Dec 5.
Published in final edited form as: Circulation. 2017 Oct 4;136(23):2271–2283. doi: 10.1161/CIRCULATIONAHA.117.030972

Figure 4. IFN- γstrongly induces MMP2 expression under IDO activation with the addition of Trp.

Figure 4

(a) Cultured HASMCs were either untreated (control; Con) or treated with IFN-γ combined with the indicated concentrations of exogenous Trp for 48 hours. (b) Cultured HASMCs were either untreated (control; Con) or treated with IFN-γ and 500µM Trp for the indicated time points. (c) HASMCs were transfected with scrambled siRNA or IDO siRNA and treated with DMSO (vehicle) or IFN-γ in the presence and absence of 500µM Trp for 48 hours. (a–c) Pro-MMP2, IDO, and GAPDH proteins were detected by immunoblotting. MMP2 activities in the culture medium were detected by zymography. Trp and Kyn levels in the culture medium were detected by high-performance liquid chromatography (HPLC). Three independent experiments for all quantitative data. The error bars in a–c are s.e.m.