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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Thromb Haemost. 2017 Nov 8;15(12):2451–2460. doi: 10.1111/jth.13869

Figure 2.

Figure 2

Silencing of PAI-1 gene expression reduces SMC VN gene expression and protein content. Murine wild-type SMCs were transfected with PAI-1 siRNA expression plasmid or negative control plasmid. Gene expression was assessed by qRT-PCR and protein content was assessed by Western blotting. (A) PAI-1 gene expression. (B) VN gene expression. (C) Representative Western blot. (D–E) Densitometric analysis of PAI-1 and VN band density, respectively. Data are mean of 3 independent experiments and are expressed as fold-control. *P<0.05 vs. control.