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. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Dev Cell. 2017 Oct 19;43(5):618–629.e5. doi: 10.1016/j.devcel.2017.09.024

Figure 1. MPK3, MPK4, and MPK6 are activated by cold treatment.

Figure 1

(A) Ten-day-old wild-type seedlings were treated at 4°C for 30 min or not treated (0 min). The relative phosphorylation levels of MPK3, MPK4, and MPK6 before and after cold treatment were determined by a quantitative phosphoproteomics assay. Data are means ± SD (n=3). Asterisks indicate statistically significant differences (**P < 0.01, Student’s t-test).

(B) Ten-day-old wild-type seedlings were treated at 4°C for 0, 5, 15, 30, and 60 min. Total proteins were extracted and immunoblotting assays were performed using anti-pTEpY, anti-MPK3, anti-MPK6, anti-MPK4, and anti-Actin.

(C) Ten-day-old wild-type seedlings were treated at 18°C, 10°C, and 4°C for 0, 15, and 30 min. The immunoblotting assays were performed using anti-pTEpY and anti-Actin. See also Figure S2 and Table S1.