REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-GFP	Sigma-Aldrich	Cat#11814460001
Rabbit polyclonal anti-MPK3	Sigma-Aldrich	Cat# M8318
Rabbit polyclonal anti-MPK6	Sigma-Aldrich	Cat# A7104
Rabbit polyclonal anti-MPK4	Sigma-Aldrich	Cat# A6979
Rabbit polyclonal anti-pTEpY	Cell Signaling Technology	Cat#9101
Mouse monoclonal anti-Actin	Agrisera	Cat#AS10 702
Rabbit polyclonal anti-MKK2	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
MG132	Sigma-Aldrich	Cat#C2211
Phos-tag	Wako	Cat#AAL-107
PerfeCTa SYBR Green Fastmix	Quanta Biosciences	Cat#95072
Dexamethasone (DEX)	Sigma-Aldrich	Cat#D4902
Glu-C	Sigma-Aldrich	Cat#11420399001
Trypsin	Promega	Cat#V5111
Leupeptin	Sigma-Aldrich	Cat#11017101001
Aprotinin	Sigma-Aldrich	Cat#10236624001
Antipain	Sigma-Aldrich	Cat#10791
Phosphatase Inhibitor Cocktail Set II	Calbiochem	Cat#524625
Protector RNase Inhibitor	Sigma-Aldrich	Cat#3335402001
Critical Commercial Assays
Gateway BP Clonase II Enzyme Mix	Thermo Fisher Scientific	Cat#11789020
Gateway LR Clonase II Enzyme Mix	Thermo Fisher Scientific	Cat#11791020
TURBO DNA-free Kit	Ambion	Cat#AM1907
M-MLV Reverse Transcriptase	Promega	Cat#M1705
Protein G Agarose	Millipore	Cat#16-266
Ni-NTA Agarose	QIAGEN	Cat#30210
Glutathione Sepharose 4B	GE Healthcare	Cat#17-0756-01
E.Z.N.A. Plant RNA Kit	Omega Bio-tek	Cat#R6827
Quick Start Bradford 1x Dye Reagent	BIO-RAD	Cat#5000205
QIAGEN Plasmid Midi Kit	QIAGEN	Cat#12143
PrimeSTAR HS DNA Polymerase	Takara	Cat#R010A
Wizard SV Gel and PCR Clean-Up System	Promega	Cat#A9282
E.Z.N.A. Plasmid Mini Kit I	OMEGA	Cat#D6942
Experimental Models: Organisms/Strains
E. coli BL21		N/A
Agrobacterium tumefaciens (strain GV3101)		N/A
Arabidopsis thaliana: WT Col-0		N/A
Arabidopsis thaliana: mpk3-1	ABRC	SALK_151594
Arabidopsis thaliana: mpk6-3	ABRC	SALK_127507
Arabidopsis thaliana: mekk1-1	(Ichimura et al., 2006)	N/A
Arabidopsis thaliana: mkk1-2	(Gao et al., 2008)	N/A
Arabidopsis thaliana: mkk2-1	(Gao et al., 2008)	N/A
Arabidopsis thaliana: mkk1-2 mkk2-1	(Gao et al., 2008)	N/A
Arabidopsis thaliana: mpk4-2	(Xing et al., 2008)	N/A
Arabidopsis thaliana: MPK6SR	(Xu et al., 2014)	N/A
Arabidopsis thaliana: MKK5DD	(Ren et al., 2002)	N/A
Arabidopsis thaliana: crlk1	(Yang et al., 2010a)	SALK_016240
Arabidopsis thaliana: crlk2	ABRC	SALK_103505
Arabidopsis thaliana: crlk1 crlk2	This study	N/A
Arabidopsis thaliana: yda	(Wang et al., 2007)	N/A
Arabidopsis thaliana: p35S::GFP-ICE1	(Chinnusamy et al., 2003)	N/A
Arabidopsis thaliana: pICE1::ICE1-GFP	This study	N/A
Arabidopsis thaliana: pICE1::ICE1-YFP	This study	N/A
Arabidopsis thaliana: pICE1::ICE1 S94A-YFP	This study	N/A
Arabidopsis thaliana: pICE1::ICE1 T366A-YFP	This study	N/A
Arabidopsis thaliana: pICE1::ICE1 S403A-YFP	This study	N/A
Arabidopsis thaliana: pICE1::ICE1 S94A T366A S403A-YFP	This study	N/A
Oligonucleotides
Primers for genotyping mutants	This study; Table S2	N/A
Primers for plasmid construction	This study; Table S2	N/A
Primers for qRT-PCR	This study; Table S2	N/A
Recombinant DNA
pET-28a HIS-MPK3	This study	N/A
pET-28a HIS-MPK6	This study	N/A
pET-28a HIS-MKK5DD	This study	N/A
pGEX-4T-1 GST-ICE1	This study	N/A
pGEX-4T-1 GST-ICE1 S94A	This study	N/A
pGEX-4T-1 GST-ICE1 S203A	This study	N/A
pGEX-4T-1 GST-ICE1 T366A	This study	N/A
pGEX-4T-1 GST-ICE1 T382A	This study	N/A
pGEX-4T-1 GST-ICE1 T384A	This study	N/A
pGEX-4T-1 GST-ICE1 S403A	This study	N/A
PMDC107 pICE1::ICE1-GFP	This study	N/A
R4pgwb540 pICE1::ICE1-YFP	This study	N/A
R4pGWB540 pICE1::ICE1 S94A-YFP	This study	N/A
R4pGWB540 pICE1::ICE1 T366A-YFP	This study	N/A
R4pGWB540 pICE1::ICE1 S403A-YFP	This study	N/A
R4pGWB540 pICE1::ICE1 S94A T366A S403A-YFP	This study	N/A
pCambia MPK3-nLUC	This study	N/A
pCambia MPK3-cLUC	This study	N/A
pCambia MPK6-nLUC	This study	N/A
pCambia MPK6-cLUC	This study	N/A
pCambia ICE1-nLUC	This study	N/A
pCambia ICE1-cLUC	This study	N/A
pHBT95 ICE1	This study	N/A
pHBT95 MPK3	This study	N/A
pHBT95 MPK6	This study	N/A
pHBT95 MPK4	This study	N/A
pHBT95 MKK5DD	This study	N/A
Software and Algorithms
ImageJ2	ImageJ	https://imagej.net/Welcome
MaxQuant	(Cox and Mann, 2008)	http://www.biochem.mpg.de/5111795/maxquant
SPSS	SPSS	https://www.ibm.com/us-en/marketplace/spss-statistics