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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Mol Carcinog. 2017 Sep 22;57(1):57–69. doi: 10.1002/mc.22731

FIGURE 1. B2G2-induces cell death in human PCa cells via increasing ROS generation.

FIGURE 1

A–B, LNCaP and 22Rv1 cells were treated with indicated doses of B2G2 for 24 and 48 h. At the end of each time point, floating and adherent cells were collected and dead cells percentage was measured. C, PWR-1E cells were treated with indicated doses of B2G2 for 24 h, and floating and adherent cells were collected and dead cells percentage was measured. D–E, LNCaP and 22Rv1 cells were treated with B2G2 (50 μM) for the indicated time points. At the end of each time point, ROS generation in terms of DCF (arbitrary fluorescence unit) was measured as described in the ‘Materials and Methods’ section. E–G, LNCaP cells were treated with NAC (10 mM) 15 min prior to B2G2 (50 μM) treatment and images were captured at the end of the experiment (24 h) and representative photographs are shown (F). In this experiment, ROS generation was measured at 6 h (G) and total cell number and percentage of cell death were determined via trypan blue assay at 24 h (H). In each case, data is expressed as mean ± SEM (n=3). * P < 0.05, significant with respect to control group; # P < 0.05, significant with respect to the B2G2-treated group.