Skip to main content
. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Cornea. 2018 Jan;37(1):95–101. doi: 10.1097/ICO.0000000000001400

Figure 2. Dry eye disease in donor promotes dendritic cell maturation in graft recipient.

Figure 2

(A) Representative in vivo confocal microscopy (IVCM) images displaying dendritic cells in the cornea of mice with DED and in healthy donors (n=10/group). The size of image is 400 × 400 μm2. (B) Number of dendritic cells, identified as bright dendritiform cells, per section in DED and healthy donor corneas (***p <0.001). (C) Representative flow cytometry plots showing mature CD11c+ MHCIIhigh dendritic cells (DCs) in cornea 14 days post-transplantation. (D) Mean frequencies of mature CD11c+ MHCIIhigh DCs among CD45+ cells in the cornea were assessed using flow cytometry (n=5, ***p<0.001). (E) Representative flow cytometry plots showing mature CD11c+ MHCIIhigh DCs in the draining lymph nodes (dLNs). (F) Mean frequencies of mature CD11c+ MHCIIhigh DCs among CD45+ cells in the dLNs were assessed using flow cytometry (n=5, *p<0.05). p values were calculated using the Student’s t-test and error bars represent standard error of mean.