FIGURE 7.

Effect of antagonists on CGS 21680-dependent pERK1/2, pJNK MAPK kinase, and pAKT activation. MRMT-1, A375, and A549 cells in the absence or in the presence of 100 nM ZM 241385 and 10 nM TP455 were exposed to 100 nM CGS for 5 min or for 30 min and pERK1/2 (A), pJNK (B), and pAKT (C) protein levels were evaluated by AlphaScreen SureFire pMAPK assays respectively. Solutions were made up in DMSO and then diluted in cell culture medium (0.1 max 0.2% of DMSO). An equal amount of DMSO was used in control cells (CTR). ^∗P < 0.01 compared with CGS 21680 100 nM. Means ± SE values from four experiments are shown. Analysis was by one-way ANOVA followed by Dunnett’s test.