Table 2.
Quantified values of discriminatory features.
| Feature | Spectral deconvolution | Amide I and II | Absorbance values | |||||
|---|---|---|---|---|---|---|---|---|
| Amide I 1600–1700 cm−1 | Mixed region 1000–1140 | Position amide I | Ratio: abs Amide I/II | νas (PO2 −) ~1212 cm−1 | C-H def. ~1335 cm−1 | |||
| Integral values | Ratio (α/β) | Integral sum | ||||||
| α-helix | β-sheet | |||||||
| Control | 40.6–44.9 | 16.3–19.3 | 2.2–2.7 | 12.2–13.9 | 1640–1645 | 1.10–1.13 | 0.42–0.44 | 0.54–0.59 |
| B16 | 38.9–40.2 | 19–20.2 | 1.9–2.1 | 14.8–16.8 | 1638–1644 | 1.10–1.15 | 0.42–0.45 | 0.54–0.59 |
| EL4 | 38.1–39.9 | 19.1–21.1 | 1.7–2.1 | 14.8–17.1 | 1636–1642 | 1.12–1.15 | 0.43–0.46 | 0.58–0.59 |
Clear separation can be seen between control and cancerous cases (both B16 and EL4) while comparing integral values of α-helix, β-sheet structure components, and their ratios. Similarly, altered position of amide I peak, amide I/amide II ratio and absorbance values at 1212 and 1335 cm−1 show significant difference only between EL4 and control.