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. 2017 Dec 5;7:16983. doi: 10.1038/s41598-017-17289-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of T-5224 on IVD degeneration of tail-punctured rats. (a) Histological grading scores of punctured discs of rats administered vehicle solution or T-5224 (total n = 32; n = 7 for both vehicle and T-5224 groups at 4 weeks, n = 9 for both vehicle and T-5224 groups at 8 weeks). Error bars denote the mean ± SD. *P < 0.05 (Student t test). (b) Safranin O staining image of a control disc. (c) Safranin O staining of half-punctured discs 8 weeks after the administration of vehicle solution or T-5224. (d) Safranin O staining of a full-punctured disc 8 weeks after the administration of vehicle solution or T-5224. Scale bars = 1 mm.