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. 2017 Dec 5;7:17027. doi: 10.1038/s41598-017-17288-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Soluble factors from CFC tissue but not from IFC tissue amplify αCD3 triggered T cell proliferation. Human CFSE-labeled PBMC were stimulated with a low dose of αCD3 antibody and subjected to CFC or IFC tissue conditioned medium (CM). After 4 days, PBMC were harvested, stained with fluorochrome-labeled human specific antibodies for T cell subset markers and analyzed by flow cytometry. Representative flow cytometry plots show the induction of CD4+ T cell (a) and CD8+ T cell (c) proliferation in PBMC cultures stimulated with either αCD3 alone (Ctrl.; left), or combined with CFC tissue CM (CFC-CM; middle) or IFC tissue CM (IFC-CM; right). Bar graphs show the levels of proliferated CD4+ T cells (b) and CD8+ T cells (d) in summary. Data are shown as the mean + SEM (n = 5–8) and analyzed with one-way ANOVA (Kruskal-Wallis test) *p < 0.05.