Table IV.
Correlation between the expression of miR-140-3p and miR-140-5p in each group, presented as mean (sd; range)
| Group | ΔCT3p | ΔCT5p | ΔΔCT | 2-ΔΔCT |
|---|---|---|---|---|
| Control | 11.93 (0.55; 10.95 to 12.69) | 9.48 (0.58; 8.57 to 10.16) | -2.45 (0.75; 1 to 3.59) | 6.12 (3.02; 2 to 12.042) |
| GA | 12.28 (0.42; 11.65 to 12.85) | 9.78 (0.44; 9.21 to 10.54) | -2.5 (0.26; 2.04 to 2.97) | 5.74 (1.07; 4.11 to 7.84) |
| RA | 12.3 (0.71; 11.38 to 13.63) | 9.92 (0.65; 9.17 to 10.87) | -2.39 (0.59; 1.13 to 3.12) | 5.59 (2.03; 2.19 to 8.69) |
| Mild OA | 13.95 (0.69; 12.82 to 15.47)* | 11.27 (0.93; 9.48 to 12.32)* | -2.68 (0.97; 1.69 to 5.4) | 8.56 (9.78; 3.23 to 42.22) |
| Moderate OA | 15.2 (1.12; 13.67 to 17.53)† | 12.21 (0.75; 10.97 to 12.96)† | -2.99 (1.13; 0.73 to 4.63) | 10.38 (7.66; 1.66 to 24.76) |
| Severe OA | 17.16 (0.86; 15.57 to 18.87)‡ | 14.65 (1.03; 12.58 to 15.74)‡ | -2.51 (0.69; 1.85 to 4.63) | 6.63 (5.25; 3.61 to 24.76) |
*
Significant difference in the marked group compared with the control, GA, RA, and severe OA groups
†
Significant difference in the marked group compared with the control, GA, RA, and severe OA groups, p < 0.05
‡
Significant difference in the marked group compared with the other groups
ΔCT3p = (CTmiR-140-3p - CTU6), ΔCT5p = (CTmiR-140-5p - CTU6), ΔΔCT = [(CTmiR-140-5p - CTU6) - (CTmiR-140-3p - CTU6)]], 2-ΔΔCT = log transformed ΔΔCT, the fold change in miR-140-5p expression normalised to U6 and relative to mR-140-3p
CT, threshold cycle; GA, arthritis due to gout; RA, rheumatoid arthritis; OA, osteoarthritis