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. 2017 Aug 7;36(48):6701–6711. doi: 10.1038/onc.2017.267

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Copyright © 2017 The Author(s)

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LSD1 inhibition leads to TFEB nuclear sublocalization. Tet-21/N (a) and SH-SY5Y (b) cells were treated with TCP or vehicle for the indicated times fixed and processed for anti-TFEB immunofluorescence and DAPI staining. (c) Tet-21/N cells were transfected with scramble (Ctrl) or siRNA against LSD1 (100 nM), fixed and stained with anti-TFEB and DAPI. (d) Tet-21/N cells were treated for 48 h with SP2509 or vehicle and then released into fresh medium for the indicated times before immunostaining with TFEB antibody and DAPI. Graphs show the ratio of TFEB nuclear/cytosolic signal intensity values average, normalized on negative and positive control samples (mean±s.d., n=200 cells). DAPI, 4',6-diamino-2-phenylindole.