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IgG ELISA on (A) 160 ng per well of DENV 1–4 Equad and (B) 150 ng per well of ZIKV Equad measured with flavivirus positive sera, CHIKV and Malaria positive and negative sera. One sample per patient was examined in two independent experiments and plotted as a mean data point indicating absorbance values. The dashed lines represent cut-offs determined by a ROC analysis with negative sera as controls.
