Table 3. Sensitivity and specificity of DENV- and ZIKV- Equad in IgM- and IgG- measurements.
| DENV Equad | ZIKV Equad | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sera | N | Positive | Sensitivity | Specificity | 95% CI | Sera | N | Positive | Sensitivity | Specificity | 95% CI |
| IgM | |||||||||||
| DENV | 54 | 49 | 90.74% | — | 79.70%–96.92% | DENV | 54 | 5 | 90.74% | 79.70%–96.92% | |
| ZIKV | 16 | 0 | — | 100% | 79.41%–100% | ZIKV | 16 | 14 | 87.5% | — | 61.65%–98.45% |
| WNV | 16 | 0 | — | 100% | 79.41%–100% | WNV | 16 | 0 | 100% | 79.41%–100% | |
| NEG | 17 | 0 | — | 100% | 80.49%–100% | NEG | 17 | 0 | 100% | 80.49%–100% | |
| Total control sera | 49 | 0 | 100.00 | 92.75%–100% | Total control sera | 87 | 5 | 94.25% | 84.12%–96.70% | ||
| IgG | |||||||||||
| DENV Tla | 9 | 9 | 100% | — | 63.37%–100% | DENV TLa | 9 | 5 | — | 44.44% | 10.70%–48.41% |
| ZIKV Tla | 12 | 5 | — | 58.33% | 18.44%–67.08% | ZIKV TLa | 12 | 12 | 100% | — | 15.70%–84.30% |
| WNV | 24 | 0 | — | 100% | 85.75%–100% | WNV | 24 | 0 | 100% | 85.75%–100% | |
| JEV | 4 | 0 | 100% | 39.76%–100% | JEV | 4 | 0 | 100% | 39.76%–100% | ||
| TBEV | 24 | 0 | — | 100% | 85.75%–100% | TBEV | 24 | 0 | 100% | 85.75%–100% | |
| CHIKV | 8 | 0 | — | 100% | 63.06%–100% | CHIKV | 8 | 0 | 100% | 63.06%–100% | |
| YFVvac | 8 | 0 | — | 100% | 63.06%–100% | YFVvac | 8 | 0 | 100% | 63.06%–100% | |
| MAL | 6 | 0 | — | 100% | 54.07%–100% | MAL | 6 | 0 | 100% | 54.07%–100% | |
| NEG | 17 | 0 | — | 100% | 80.49%–100% | NEG | 17 | 0 | 100% | 80.49%–100% | |
| Total control sera | 103 | 5 | 95.15% | 89.03%–98.41% | Total control sera | 100 | 5 | 95% | 88.72%–98.36% | ||
| IgGcomp | |||||||||||
| DENV TLb | 23 | 23 | 100% | — | 85.18%–100% | DENV TLb | 23 | 0 | — | 100% | 85.18%–100% |
| DENV END | 55 | 55 | 100% | — | 93.51%–100% | DENV END | 55 | 2 | — | 96.36% | 87.47%–99.56% |
| ZIKV TLb | 18 | 1 | — | 94.44% | 73.97%–99.87% | ZIKV | 18 | 18 | 100% | — | 81.47%–100% |
| (ZIKV END)a | 21 | 18 | — | 14.29% | 3.05%–36.34% | ZIKV END | 21 | 21 | 100% | — | 83.89%–100% |
| NEG | 17 | 0 | — | 100% | 80.49%–100% | NEG | 17 | 0 | 0 | 100% | 80.49%–100% |
| Total control sera | 35 | 1 | 97.14% | 85.08%–99.93% | Total control sera | 95 | 2 | 97.89% | 92.60%–99.74% | ||
Abbreviation: Confidence interval, CI, refers to the number of samples in the cohort. Bold entries represent sera groups which are infected with a to the antigen homologous virus and also total specificites of each test are highlighted in bold.
a
Group was excluded from specificity measurements because of 90% DENV seroprevalence.