Table 1. Detection of viral strain compositions in standard mixtures.
Sample | Expected proportion | Observed composition (CBS) | Observed composition (TGS) | ||
---|---|---|---|---|---|
Number of strains (total) | Proportion | Number of strains (total) | Proportion | ||
C01 | 0.80/0.20 | 15/3 (19) | 0.79/0.16 | 552/137 (697) | 0.79/0.20 |
C02 | 0.50/0.50 | 9/9 (19) | 0.47/0.47 | 374/370 (747) | 0.50/0.50 |
C03 | 0.20/0.80 | 5/15 (21) | 0.24/0.71 | 157/629 (799) | 0.20/0.79 |
Abbreviations: clone-based sequencing, CBS; third-generation sequencing, TGS.