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. 2017 Dec 5;7:16992. doi: 10.1038/s41598-017-17287-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Colocalization of cytochrome c and mitochondria in blastocysts in different groups. (A) Blastocysts were labeled with a cytochrome c specific antibody (green) and Mito Tracker Red. (B) Colocalization was analyzed using Image-Pro Plus; frame indicates released cytochrome c. Pearson’s correlation was analyzed using Image-Pro Plus. Data are expressed as the mean ± SEM. In addition, ***p < 0.001 indicates significant differences between different groups. Bar = 40 µm. The experiments were replicated three times with 246 blastocysts.