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Primer sequences used in real time RT-PCR.
| Gene | Primer sequence (5′-3′) | Product Size (bp) |
|---|---|---|
| COX2 | F:GGCTGCGGGAACATAATAGA | 183 |
| R:GCAGCTCTGGGTCAAACTTC | ||
| FN1 | F:AGGGCGATGAACCACAGT | 221 |
| R:GCTCCAGCGAACGACAAT | ||
| ITGA5 | F:TGATGACAGTTATTTGGGCTAC | 100 |
| R: CAAAGTCCTCGCTGCTCT | ||
| GAPDH | F:TTCCACGGCACAGTCAAG | 117 |
| R:ATACTCAGCACCAGCATCG |
F: forward; R: reverse.
