Table 1.
PCR primers used for species-specific marker and NIV chemotype identification, target species/gene, and sequence
| Primer designation | Target gene/species | 5′ > 3′ sequence |
|---|---|---|
| FaF | F. avenaceum | AGCATTGTCGCCACTCTC |
| FaR | GTTTGGCTCTACCGGGACTG | |
| Fc01F | F. culmorum | ATGGTGAACTCGTCGTGGC |
| Fc01R | CCCTTCTTACGCCAATCTCG | |
| Fg16F | F. graminearum | CTCCGGATATGTTGCGTCAA |
| Fg16R | GGTAGGTATCCGACATGGCAA | |
| Ef 728 M | Translation elongation factor 1α (tef-1α) | CATCGAGAAGTTCGAGAAGG |
| Tef1R | GCCATCCTTGGAGATACCAGC | |
| Tri7F | Nivalenol (NIV) chemotype | ATCGTGTACAAGGTTTACG |
| Tri7NIV | TTCAAGTAACGTTCGACAAT |