Fig. 4.

Impaired voluntary motor performance in Ano2 ^−/− mice. a Both wild-type (black) and Ano2 ^−/− mice (red) used running wheels for nocturnal activity. The shaded boxes depict the dark periods from 7 pm to 7 am during the 84-h experiment. Plotted are the rounds accumulated over successive 2-h intervals. b Both mouse lines used the wheel for similar durations within the active period, proving comparable motivation for the task. c Over the four nights of activity, Ano2 ^−/− mice ran on average fewer rounds on the wheel than wild-type animals (wt 2599 ± 336 rotations/2 h; Ano2 ^−/− 939 ± 95.6 rotations/2 h; two-way ANOVA, p < 0.001, Bonferroni correction). d The motor performance on the running wheel increased in wild-type but not in Ano2 ^−/− mice. Two-way ANOVA: genotypes, p < 0.001; time dependence, p = 0.11; interaction, p = 0.2. Mean values from four wild-type and five Ano2 ^−/− mice are displayed ± SEM; ***p < 0.001