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. 2017 Dec 5;7:16945. doi: 10.1038/s41598-017-16095-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM of CLas and ER dynamics in D. citri infected gut cells. (A) Dense RER in healthy midgut epithelia cell. (B) Rearrangement of rough ER (RER) membranes around the light matter (LM) inside a midgut cell. (C) Liberibacter containing vacuoles (LCVs) showing the LM and the dark matter (DM) inside them. LCVs are located close to midgut basal lamina. Two adjacent LCVs are shown in the inset. LCV borders are indicated with arrows. (D) Zoom-in on one LCV showing mature CLas bacteria (B) in the LM, surrounded by the double membranous ER (arrows).